糖心视频

How do some proteins survive the extreme heat generated when they catalyze reactions that can happen as many as a million times per second? Work by researchers from Indiana University-Purdue University Indianapolis (IUPUI) and the University of California Berkeley published online on Dec. 10 in Nature provides an explosive answer to this important question.

Proteins are essential to the human body, doing the bulk of the work within cells. Proteins are large molecules responsible for the structure, function, and regulation of tissues and organs. Enzymes鈥攕pecial proteins that catalyze chemical reactions within cells鈥攁re critical to every bodily function from breathing to walking. Some enzymes produce a lot of heat per reaction. Enough heat, in fact, that if that heat were to be injected in another protein, that protein would overheat and unfold. So, how do enzymes expel that heat without overheating and self-destructing?

Steve Press茅, Ph.D., assistant professor of physics in the School of Science at IUPUI, led the study's theoretical arm. He is co-corresponding author of the Nature study by Riedel et al. along with Howard Hughes Medical Institute investigator Carlos Bustamante, Ph.D., of the University of California Berkley, who led the experimental research arm of the study in close collaboration with Susan Marqusee, M.D., Ph.D., also of UC Berkeley.

"A critical goal in improving human health will be to understand how a protein recovers from a reaction and, ultimately, how to speed up its activity," said Press茅, a biophysicist at IUPUI.

"We have discovered a key fact that explains how enzymes recover from a reaction: enzymes dissipate heat by very rapidly accelerating immediately following the reaction. This finding has very deep implications regarding how heat flows in living systems."

To illustrate how this heat transfer appears to occur, Press茅 refers to an observation made by Alexander Graham Bell in the 19th century, which lead to the discovery of the 'photoacoustic effect'. Noting that metal鈥攚hen exposed to sun then to shade鈥攅mitted a ringing sound, Bell concluded that heat from light expanded the metal that then recontracted in the shade. In doing so, the metal sent audible pressure waves out into the air.

Similarly, Press茅 explains, enzymes respond to the energy released during catalytic reactions by expanding and contracting which in turn violently propels the enzyme and generates a pressure wave鈥攖he study authors call it a chemoacoustic wave鈥攂ecause it is caused by the heat of a chemical reaction.

"Think of proteins as stepping on landmines. We asked how does a protein avoid damage from the enormous amounts of heat released and not break apart? Now we have shown that they cope with this heat assault by pushing that energy outwards from the reaction site as chemoacoustic waves and propelling themselves away in the meanwhile," said Press茅.

The Press茅, Bustamante and Marqusee labs plan to continue investigating this puzzling 'chemoacoustic effect' on a number of other proteins using a variety of experimental and theoretical methods.