糖心视频

The epithelial cells lining organs like the intestines and kidneys build a special surface called the "brush border," which consists of a dense array of finger-like protrusions.

Irina Kaverina, Ph.D., Matthew Tyska, Ph.D., and colleagues in Argentina explored the role of microtubules鈥攑art of the cellular "skeleton"鈥攊n building the border, which is critical for healthy organ function.

The investigators used a cell model of individual intestinal cell polarization (the establishment of "sidedness") and found that disruption of microtubules prevented brush border formation. They confirmed the findings in cultured kidney cells.

They demonstrated that the microtubules essential for brush border development derived from the centrosome, not the Golgi, and that overexpression of a protein associated with microtubule plus ends facilitated brush border formation.

The findings, reported in the February issue of the Journal of Cellular 糖心视频iology, reveal a role for microtubules in the organization of the brush border and a novel mechanism of microtubule regulation of epithelial polarity.