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When it comes to processing coffee beans, longer fermentation times can result in better taste, contrary to conventional wisdom. Lactic acid bacteria play an important, positive role in this process. Other species of microbes may play a role in this process as well, but more research is needed to better understand their role. The research is published February 1 in Applied and Environmental Microbiology.

"A cup of coffee is the final product of a complex chain of operations: farming, post-harvest processing, roasting, and brewing," said principal investigator Luc De Vuyst, M.Sc., Ph.D., Professor of Industrial Microbiology and Food Biotechnology, Vrije Universiteit Brussel, Brussels, Belgium. "There are several variants of post-harvest processing, among which wet processing and dry processing are the most common." Wet processing鈥攃ommonly used for Arabica and specialty coffees鈥攊s the step that includes fermentation.

"We carried out the research at an experimental farm in Ecuador through a multiphasic approach, encompassing microbiological, metabolomics, and sensory analysis," said Dr. De Vuyst.

Fermentation was of particular importance. During extended fermentation, leuconostocs鈥攁 genus of lactic acid bacteria used in the fermentation of cabbage to sauerkraut and in sourdough starters鈥攄eclined in favor of lactobacilli, said Dr. De Vuyst. Lactic acid bacteria were already present before fermentation, and these acid tolerant lactobacilli proliferated even more during this process.

However, it is challenging to draw a causal link between the microbiota and the volatile compounds in the beans鈥攖hose compounds that contribute to the coffee's smell鈥攕ince many of these compounds can be of microbial, endogenous bean metabolism, or chemical origin," said Dr. De Vuyst.

"However, we did see an impact of the microbial communities, in particular the lactic acid bacteria," said Dr. De Vuyst. They yielded fruity notes, and may have "had a protective effect toward coffee quality during fermentation because of their acidification of the fermenting mass, providing a stable microbial environment and hence preventing growth of undesirable micro-organisms that often lead to off-flavors," he said.

"Furthermore, there is a build-up of the fermentation-related metabolites onto the coffee beans, which affects the quality of the green coffee beans and hence the sensory quality of the coffees brewed therefrom," said Dr. De Vuyst.

Dr. De Vuyst emphasized that how each stage of processing influences the taste of coffee remains mostly uncharted. "We were aware of many different micro-organisms during wet coffee fermentation鈥攅nterobacteria, lactic acid bacteria, yeasts, acetic acid bacteria, bacilli, and filamentous fungi," said Dr. De Vuyst, but it is still unknown how most bacteria influence this process.

The work was a collaboration between the Vrije Universiteit Brussel, and the Nestl茅 Research Center. "Nestl茅 was interested in the fundamental aspects of coffee processing, in particular, the post-harvest processing chain, in order to correlate it with the roasting process and of course the final cup quality," said Dr. De Vuyst.